Site Directed Mutagenesis (SDM) Human Deletion MDA-MB-231

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Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Human - HO-1

Products R&D Systems Human Total HO-1/HMOX1 DuoSet IC ELISA

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells FE002-SK2 human skin progenitor cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells MCF-7 human breast cancer cells

Get tips on using Tau Protein Ladder, 6 isoforms human to perform Protein Ladder Immunofluorescence

Products Sigma-Aldrich Tau Protein Ladder, 6 isoforms human

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Products STEMCELL technologies RosetteSep™ Human Monocyte Enrichment Cocktail

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Products STEMCELL technologies EasySep™ Human Monocyte Enrichment Kit

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Products STEMCELL technologies EasySep™ Human Monocyte Isolation Kit

Get tips on using Human Notch-1 Intracellular Domain Antibody to perform Western blotting Notch1

Products R&D Systems Human Notch-1 Intracellular Domain Antibody

Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF

Products Aviscera Bioscience Active BDNF (Human, Rat) ELISA Kit

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

RNA siRNA / RNAi /miRNA transfection Human Cells HT-1376 GLUT1

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