siRNA / miRNA gene silencing Rat Brain endothelial cells

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

Get tips on using PE-Cy™7 Rat Anti-Mouse TNF to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using Alexa Fluor® 488 Rat Anti-Mouse CD146 to perform Flow cytometry Anti-bodies Mouse - CD146/MCAM

Get tips on using PE-Cy™7 Rat anti-Mouse CD117 to perform Flow cytometry Anti-bodies Mouse - CD117/c-kit

Get tips on using PE-Cy™7 Rat Anti-Mouse CD31 to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using miRNA Complete Labeling and Hyb Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp