Protein expression and purification Mammalian cells

- Found 9484 results

RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile

RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

RNA isolation / purification Bacteria - Gram positive Clostridum botulinum Experiment

RNA RNA isolation / purification Bacteria Gram positive Clostridum botulinum

RNA isolation / purification Bacteria - Gram positive Lactobacillus amylovorus Experiment

RNA RNA isolation / purification Bacteria Gram positive Lactobacillus amylovorus

RNA isolation / purification Bacteria - Gram positive Listeria monocytogens Experiment

RNA RNA isolation / purification Bacteria Gram positive Listeria monocytogens

RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus epidermidis

RNA isolation / purification Bacteria - Gram positive Staphylococcus saprophycitius Experiment

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus saprophycitius

RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes Experiment

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pyogenes

ChIP Mouse - 3T3-L1 cells Experiment

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Mouse 3T3-L1 cells

Protein enrichment Soluble nuclear proteins Experiment

Proteins Protein enrichment Soluble nuclear proteins

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