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4 years ago
4 years ago by Paul G. Macon
Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?
Found 2 matching solutions for this experiment
|Total RNA was extracted from the lysates by an InviMag Universal RNA Kit (Stratec) using the KingFisher Flex (Thermo Scientific, Langenselbold, Germany) according to the manufacturer’s instructions. The yield of total RNA was estimated spectrophotometrically at 260 nm and isolated RNA was stored at -80°C. RNA quality and integrity was verified using the Agilent 2100 Bioanalyzer (RNA 6000 Nano Chip, Agilent, Waldbronn, Germany).|
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