rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Blood / Serum / Plasma / Buffy coat

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized OS-RC-2

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using XcelGen Plant RNA Isolation Mini Kit to perform RNA isolation / purification Plants - Seeds

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized Mono-Mac-6

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue