DNA transfection Mammalian cells Primary cells

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Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - OUMS-27

Products Thermo Fisher Scientific Pierce™ Coomassie (Bradford) Protein Assay Kit

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - Rat_Liver

Products Thermo Fisher Scientific NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - HEK293T

Products Thermo Fisher Scientific NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Human Glioblastoma stem-like cells (GSCs)

Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - HEK 293

Products Thermo Fisher Scientific LIVE/DEAD Fixable Violet Dead Cell Stain Kit

Get tips on using LIVE/DEAD Fixable Violet Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Products Thermo Fisher Scientific LIVE/DEAD Fixable Violet Dead Cell Stain Kit

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human CAL-27 DPAGT1

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

Products PromoKine Live/Dead cell Staining Kit II

Get tips on using LIVE/DEAD™ Cell Imaging Kit to perform Live / Dead assay mammalian cells - SH-SY5Y Human neuroblastoma

Products Thermo Fisher Scientific LIVE/DEAD™ Cell Imaging Kit

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - bmMSCs human bone marrow

Products PromoKine Live/Dead cell Staining Kit II

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