siRNA / miRNA gene silencing Human CRL-5915

Get tips on using Human BDNF PicoKine™ ELISA Kit to perform ELISA Human - BDNF

Get tips on using Adiponectin Human ELISA, High Sensitivity (Sandwich) to perform ELISA Human - Adiponectin

Get tips on using Classical Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using VWF (Human) ELISA Kit to perform ELISA Mouse - vWF-A2

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Human VE Cadherin ELISA Kit (ab210968) to perform ELISA Human - VE Cadherin

Get tips on using Human VE-Cadherin Quantikine ELISA Kit to perform ELISA Human - VE Cadherin

Get tips on using Human TNF alpha ELISA Kit (ab181421) to perform ELISA Human - TNF-alpha

Get tips on using Human TNF-alpha Quantikine ELISA Kit to perform ELISA Human - TNF-alpha

Get tips on using Human PDGF BB ELISA Kit (ab100624) to perform ELISA Human - PDGF-BB