Found 2 discussions for this experiment
4 years ago
4 years ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
4 years ago
4 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 3 matching solutions for this experiment
|Whole lung tissue was homogenized prior to RNA extraction (BeadBlaster 24, Benchmark Scientific) in the presence of lysis buffer (RLT Plus, Qiagen). Total RNA from whole lung tissue and sorted alveolar macrophages and alveolar type II cells was extracted (AllPrep DNA/RNA Mini Kit, Qiagen) and quality and quantity were assessed (TapeStation 4200, Agilent).
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