Site Directed Mutagenesis (SDM) Mouse Deletion 3T3-L1

Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mouse aortic endothelial cells (MAECs)

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - mouse splenocytes

Get tips on using FlexiTube GeneSolution GS27279 for Tnfrsf12a to perform siRNA / miRNA gene silencing Mouse - B16-BL6 FN14/Tnfrsf12a

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using Anti-Atg5 to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using INTERFERin® to perform siRNA / RNAi /miRNA transfection Mouse - B16 Polymer / lipid