Get tips on using High-capacity cDNA reverse transcription kit to perform cDNA synthesis Cell lines
Get tips on using REPLI-g UltraFast Mini Kit (100) to perform Whole Genome Amplification Cell lines
Get tips on using EmbryoMax MEF Conditioned Media to perform Stem cell culture media hESC lines H9, H1
Get tips on using cDNA SynthePrimeScript™ 1st strand ssynthesis Kit to perform cDNA synthesis Cell lines
Get tips on using M-MLV Reverse Transcriptase (200 U/µL) to perform cDNA synthesis Cell lines
Get tips on using ChIP-IT® Express Chromatin Immunoprecipitation Kits to perform ChIP Human - Fibroblast cell lines
Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Cell lines
RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.
Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage
Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage
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