Autophagy assay

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Cell Meter™ Autophagy Assay Kit *Green Fluorescence to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - HT144

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - J3

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - REH

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - Cholangiocarcinoma

Get tips on using Autophagy Inhibitor, 3-MA to perform Autophagy assay cell type - LN229