DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.
Get tips on using DNA-spin™ Plasmid DNA Purification Kit to perform Plasmid Isolation Enterobacteriaceae
Get tips on using Bone DNA Purification Kit to perform DNA isolation / purification Tissue - bone
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Salmonella enterica
Get tips on using Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
Get tips on using PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit to perform Cell Culture Contamination Detection Kit Mycoplasma
Get tips on using PCR SuperMix to perform PCR Conventional / Qualitative PCR - mammalian DNA
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