siRNA / miRNA gene silencing Rat INS-1

Get tips on using COL1A Antibody (COL-1): sc-59772 to perform Western blotting Type I collagen

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - CAMs

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Hepatocytes

Get tips on using Anti-Beclin 1 antibody [2A4] (ab114071) to perform Autophagy assay cell type - Saos

Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1

Get tips on using AMPIPROBE® Candida 1 Assay Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using Hop Antibody (E-1): sc-398703 to perform Flow cytometry Anti-bodies Mouse - HOPX

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase