siRNA / RNAi /miRNA transfection Human Jurkat cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using PE Mouse Anti-Human CD140a to perform Flow cytometry Anti-bodies Human - CD140/PDFGR2

Get tips on using PE Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using APC Mouse Anti-Human CD184 to perform Flow cytometry Anti-bodies Human - CD184/CXCR4

Get tips on using FITC Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38

Get tips on using Purified Mouse Anti-Human CD36 to perform Flow cytometry Anti-bodies Human - CD36/CB38

Get tips on using FITC Rat Anti-Human CD49f to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using BUV395 Mouse Anti-Human CD123 to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using PE Mouse Anti-Human CD31 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using Human IL-6R alpha Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha