siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

Get tips on using Mouse Lipocalin-2/NGAL PicoKine™ ELISA Kit to perform ELISA Mouse - NGAL/LCN2

Get tips on using Mouse Von Willebrand Factor A2 ELISA Kit (ab208980) to perform ELISA Mouse - vWF-A2

Get tips on using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit to perform ELISA Mouse - RANK L

Get tips on using Mouse heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Mouse - HO-1

Get tips on using Q-Plex™ Mouse Cytokine – Inflammation (14-plex) to perform ELISA Mouse - GM-CSF

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.