sirna-rnai-mirna-transfection-human-cells-kg-1-lipofectamine

Get tips on using CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Mouse Sca-1/Ly6 APC-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines COS7

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Mouse - TGF-beta 1

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1

Get tips on using β-2-Microglobulin Antibody (BBM.1): sc-13565 to perform Western blotting β₂ microglobulin

Get tips on using Recombinant Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) to perform Western blotting CDKN1B

Get tips on using Recombinant Anti-Integrin beta 1 antibody [EPR16895] (ab179471) to perform Western blotting ITGB1