siRNA / miRNA gene silencing Rat MTLn3 (rat mammary adenocarcinoma breast cancer cell line)

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat brain tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat spleen tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using hCas9 to perform CRISPR Rat - Activation CD2

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary rat lymph node cells

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Tissue - Rat Ovaries

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Retina