Found 1 discussion for this experiment
2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 2 matching solutions for this experiment
|Fix cells in fixation solution for 1 h at +15 to +25°C.
Add tunel reaction mxture and incubate for 60 min at +37°C in a humidified atmosphere in the dark.
|If Nonspecific labeling is noticed, try formalin or glutaraldehyde; reduce concentration of TdT by diluting it 1:2 up to 1:10 with TUNEL Dilution Buffer.
If high background, reduce concentration of labeling mix
to 50% by diluting with TUNEL Dilution Buffer
If Low labeling is noticed, increase incubation time. or incubate at higher temperature
(e.g., +15 to +25°C).
Analyse using a 520–550 nm or 330–385 nm excitation filter, and a 580~ nm or 420~ nm