Protein expression and purification Mammalian cells HeLa

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using DNA-Prep Reagents Kit, RUO to perform Cell cycle assay human - HeLa

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using SuperSignal™ Enhanced Molecular Weight Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained High Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Broad Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Low Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using Unstained Protein Ladder (10 - 200 kDa) (ab234618) to perform Protein Ladder Unstained