siRNA / miRNA gene silencing Human T47-D

Get tips on using APC anti-human CD326 (EpCAM) Antibody to perform Flow cytometry Anti-bodies Human - CD326/EpCAM

Get tips on using APC anti-human/mouse CD49f Antibody to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - T47D

Get tips on using Human Candida Albicans ELISA Kit to perform Cell Culture Contamination Detection Kit Yeast

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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.