DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using E.Z.N.A.® BAC/PAC DNA-kits to perform Plasmid Isolation Acinetobacter towneri

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - rat kidney and pancreas tissue

Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse kidney tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform AAA for reviews