siRNA / miRNA gene silencing Mouse Embryonic stem cells

Get tips on using HiPerFect Transfection Reagent (100 ml) to perform siRNA / RNAi /miRNA transfection Bovine - monocyte-derived macrophages

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - AR42J Lipid based

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - IEC-6 Lipofectamine

Get tips on using Microglia Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using Adipogenic Induction Medium to perform Stem cell Differentiation media mPericytes differentiation into adipogenic cells

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - Hippocampal neural stem cells

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media hMyoblasts cells

Get tips on using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - U87 and U251 glioblastoma cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using CytoTune™-iPS 2.0 Sendai Reprogramming Kit to perform Stem cell Differentiation media Differentiation of RPE cells into hiPSC cells