RNA isolation / purification Cells Cancer cell lines

Get tips on using in situ Cell Death Detection Kit, POD to perform TUNEL assay cell type - PANC-1 human pancriatic cancer

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - PC-3 human prostate cancer

Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus epidermidis

Get tips on using Direct-zol™ RNA MiniPrep Plus to perform RNA isolation / purification Bacteria - Gram negative Borrelia burgdorferi

Get tips on using Direct-zol™ RNA MiniPrep Plus to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using PolyMag Neo to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HEK293

Get tips on using Viromer® RED to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa