Protein expression and purification Mammalian cells HEK 293

Get tips on using Mouse C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Mouse - C-Reactive Protein/CRP

Get tips on using ELISA Kit for C Reactive Protein (CRP) to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using Human C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Human - C-Reactive Protein/CRP

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Scheffersomyces (Pichia) stipitis

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse prostate tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using anti-p62 Protein, C-Terminal Specific Polyclonal Antibody to perform Autophagy assay cell type - MDA-MB-231

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.