Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris Bacteria

Get tips on using Zombie Aqua™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - human peripheral blood mononuclear cells

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Mouse - Deletion B16-F1 β2m

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using XcelGen Blood gDNA Mini Kit to perform Whole Genome Amplification Human

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media DuCaP

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media DU145

Get tips on using Advanced RPMI 1640 Medium to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)