rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Tissue - Human Joints

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Double-negative T Cell Isolation Kit, human to perform Cell Isolation Double-negative T Cell Isolation

Get tips on using RNeasy DSP FFPE Kit to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Thyroid gland

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Submandibular glands

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Lymph node

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Adrenal glands

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - human liver tissue