ChIP H3K27me3 Human Donkey

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using ChIPAb+ Trimethyl-Histone H3 (Lys36) - ChIP Validated Antibody and Primer Set to perform ChIP Anti-bodies H3K36me3

Get tips on using Anti-Rad51 antibody - ChIP Grade (ab176458) to perform ChIP Anti-bodies RAD51

Get tips on using Anti-Mre11 antibody - ChIP Grade (ab12159) to perform ChIP Anti-bodies MRE11

Get tips on using Anti-CTCF antibody - ChIP Grade (ab70303) to perform ChIP Anti-bodies CTCF

Get tips on using Anti-Sall4 antibody - ChIP Grade (ab29112) to perform ChIP Anti-bodies Sall4

Get tips on using Anti-HDAC1 antibody - ChIP Grade (ab7028) to perform ChIP Anti-bodies HDAC1

Get tips on using Magna ChIP™ G Tissue Kit to perform ChIP Mouse - Cardiac fibroblasts

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K36Me3 - Sheep Rat -NA-

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) to perform ChIP Anti-bodies H3K36me3