Get tips on using EpiTect MSP Kit to perform PCR Methylation specific PCR - Mammalian DNA
Get tips on using m6a rna methylation quantification kit to perform RNA quantification Coloremetric
Get tips on using EpiTect MethyLight PCR +ROX Vial Kit (200) to perform PCR Methylation specific PCR - Mammalian DNA
Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HEK293T
Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T
Get tips on using TruSeq Small RNA Library Preparation Kit to perform RNA sequencing Human - HEK293T
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Human - HEK293T
Get tips on using 24-Well Haptotaxis Assays, Collagen I to perform Cell migration / Invasion cell type - HEK293T
Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - HEK293T
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
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