DNA methylation profiling Gene specific profiling Rat whole pituitary glands

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RNA siRNA / miRNA gene silencing Mouse Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

RNA siRNA / miRNA gene silencing Human Jurkat MK2 (MAPK Kinase 2) Viral vectors

RNA siRNA / miRNA gene silencing Human U937 MK2 (MAPK Kinase 2) Viral vectors

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

RNA siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4 Polymer / Lipid

RNA siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1 Lipid

Get tips on using GeneArtâ„¢ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Rat - Point mutation Rat-2 PIK3CB

Products Thermo Fisher Scientific GeneArtâ„¢ Site-Directed Mutagenesis PLUS System

RNA siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

DNA CRISPR Human Repression HBV RT

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