Site Directed Mutagenesis (SDM) Human Point mutation SH-SY5Y

Get tips on using Human Total HO-1/HMOX1 DuoSet IC ELISA to perform ELISA Rat - HO-1

Get tips on using Monoclonal Mouse Anti-Human Actin (Smooth Muscle) (Concentrate) Clone 1A4 to perform Immunohistochemistry Mouse - SMA

Get tips on using EasySep™ Human Naïve B Cell Enrichment Kit to perform Cell Isolation Naive B cell

Get tips on using MojoSort™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using EasySep™ Human Naïve B Cell Isolation Kit to perform Cell Isolation Naive B cell

Get tips on using MACSprep™ HLA B Cell Isolation Kit, human to perform Cell Isolation HLA B Cell

Get tips on using MACSprep™ HLA T Cell Isolation Kit, human to perform Cell Isolation HLA T Cell

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.