Bacterial cell culture media

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human aortic endothelial cells

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Islets of langerhans (Beta cells)