ChIP H3K27me3 Rat Bovine

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Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line) SIRT1

Get tips on using CD43 antibody | DFT-1 to perform Flow cytometry Anti-bodies Human - CD43

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Get tips on using CD38 Monoclonal Antibody (HIT2) to perform Flow cytometry Anti-bodies Human - CD38

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Get tips on using Anti-Vimentin antibody (ab137321) to perform Flow cytometry Anti-bodies Human - Vimentin

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Get tips on using CD68 antibody | FA-11 to perform Flow cytometry Anti-bodies Mouse - CD68

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Get tips on using CD200 antibody | OX-90 to perform Flow cytometry Anti-bodies Mouse - CD200

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Get tips on using Purified Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

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Get tips on using PE Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

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Get tips on using PE Mouse Anti-Human CD122 to perform Flow cytometry Anti-bodies Human - CD122

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Get tips on using PE Mouse Anti-Human CD116 to perform Flow cytometry Anti-bodies Human - CD116

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