Site Directed Mutagenesis (SDM) Mouse C2C12

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Get tips on using Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker to perform Immunohistochemistry Mouse - PHH3

Products Sigma-Aldrich Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Get tips on using truChIP Ultra-Low Chromatin Shearing Kit with Formaldehyde to perform ChIP Mouse - Osteoblasts

Products Covaris truChIP Ultra-Low Chromatin Shearing Kit with Formaldehyde

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Ovary

Products Merck Millipore EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit

Get tips on using EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit to perform ChIP Mouse - Podocyte

Products Merck Millipore EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit

Get tips on using EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit to perform ChIP Mouse - HSC

Products Merck Millipore EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse small intestinal organoids

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

Get tips on using HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2 to perform shRNA gene silencing Mouse - FL83B HtrA2

Products Sigma-Aldrich HTRA2 MISSION shRNA Lentiviral Transduction Particles HtrA serine peptidase 2

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality hot-start DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction

DNA PCR Hot start PCR Bacterial DNA

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Products Beyotime Senescence β-Galactosidase Staining Kit - Beyotime

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

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