Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - J774A.1
Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Neuro 2a
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Neuro 2a
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Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human podocytes
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HK-2
Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - HK-2
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Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.
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