Cell migration / Invasion cell type - MDA-MB-231

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

ORIS™ Cell Migration Assay

Platypus Technologies

Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 4 to 18 hours (cell line dependent) to permit cell attachment.
Upstream tips
Seed 5 × 105 cells
Protocol tips
Cover the plate and transfer to a cell culture incubator for 24 hours
Downstream tips
Read the fluorescence with a fluorescence plate reader at 560 nm.
Protocol tips
Add 30% FBS medium as chemoattractant.

Incubate at 37C in a CO2 incubator for 6 hours
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