ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

Get tips on using Qubit RNA BR Assay Kit to perform RNA quantification Coloremetric

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse kidney

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

Get tips on using STEMdiff™ Hematopoietic Kit to perform Stem cell Differentiation media hiPSCs differentiation into CD43+ primitive hematopoietic progenitor cells (HPCs)

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using TACS Annexin V-FITC Apoptosis Detection Kit to perform Necrosis MCF7

Get tips on using Annexin V-FITC Kit, 200 Tests, RUO to perform Necrosis MCF7

Get tips on using XcelGen Blood gDNA Mini Kit to perform Whole Genome Amplification Human