Immunohistochemistry CD3 Mouse

Get tips on using PE anti-human CD135 (Flt-3/Flk-2) Antibody to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion CD38

Get tips on using Epithelial Antigen, Ber-EP4, Unconjugated to perform Flow cytometry Anti-bodies Human - CD326/EpCAM

Get tips on using Human Syndecan-1 ELISA Kit (CD138) (ab46506) to perform ELISA Human - SDC1

Get tips on using MojoSort™ Human B Cell (CD43-) Isolation Kit to perform Cell Isolation B cell

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).