Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using siGENOME Rat Epor siRNA to perform siRNA / miRNA gene silencing Rat - H19-7 EpoR
Get tips on using MagAttract PowerMicrobiome DNA/RNA EP to perform DNA isolation / purification Bacteria - Gram negative Klebsiella
Get tips on using DMEM, no glucose, no glutamine, no phenol red to perform 3D Cell Culture Media hiPSC-derived cardiac organoids
Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain
Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF
Get tips on using Rat VEGF Quantikine ELISA Kit to perform ELISA Rat - VEGF
Get tips on using Rat prolactin,PRL ELISA Kit to perform ELISA Rat - PRL
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