rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

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Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - human kidney tissue

Products Fisher Scientific Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Rat fibroblast-like synoviocytes

Get tips on using siGENOME Rat Epor siRNA to perform siRNA / miRNA gene silencing Rat - H19-7 EpoR

Products Dharmacon siGENOME Rat Epor siRNA

Get tips on using MagAttract PowerMicrobiome DNA/RNA EP to perform DNA isolation / purification Bacteria - Gram negative Klebsiella

Products Qiagen MagAttract PowerMicrobiome DNA/RNA EP

Get tips on using DMEM, no glucose, no glutamine, no phenol red to perform 3D Cell Culture Media hiPSC-derived cardiac organoids

Products Thermo Fisher Scientific DMEM, no glucose, no glutamine, no phenol red

Get tips on using AllPrep DNA/RNA/Protein Mini Kit (50) to perform DNA isolation / purification Tissue - brain

Products Qiagen AllPrep DNA/RNA/Protein Mini Kit (50)

Proteins Protein expression and purification Mammalian cells HEK 293 Mt-PA

Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF

Products Abcam Rat VEGF ELISA Kit (ab100786)

Get tips on using Rat VEGF Quantikine ELISA Kit to perform ELISA Rat - VEGF

Products R&D Systems Rat VEGF Quantikine ELISA Kit

Get tips on using Rat prolactin,PRL ELISA Kit to perform ELISA Rat - PRL

Products Cusabio Rat prolactin,PRL ELISA Kit

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