An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Aspc-1
Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - Bxpc-3
Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - Sao2-2, MG-62
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - CHO-K1
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - Saos-2
Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - PC-9
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - SKOV-3
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - OVCAR-3
Get tips on using Cell Proliferation Kit II (XTT) to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y
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