Protein expression and purification Bacteria Escherichia coli

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Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Products Abcam Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

Get tips on using pwPICZalpha-DT390-bi-pIL-2-Non-N-Gly to perform Protein Expression Eukaryotic cells - P. pastoris Porcine IL-2 fusion toxins

Products Zhirui Wang, Transplantation Biology Research Center, Massachuse pwPICZalpha-DT390-bi-pIL-2-Non-N-Gly

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size < 15Kb

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. The resulting amplicons are generally detected by gel electrophoresis and for some further applications like cloning, sequencing, amplicon product needs to be recovered from the gel and subsequently purified. However, non-specific product amplification and primer-dimer formation during set-up make gel extraction difficult. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

DNA DNA gel extraction / PCR product purification Product size > 15Kb

Proteins Protein isolation Tissue Human tissue C-MFPE samples

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus paracasei

Products Merck Millipore De Man, Rogosa and Sharpe (MRS) Broth

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus helveticus

Products Merck Millipore De Man, Rogosa and Sharpe (MRS) Broth

Get tips on using De Man, Rogosa and Sharpe (MRS) Broth to perform Bacterial cell culture media Lactobacillus plantarum

Products Merck Millipore De Man, Rogosa and Sharpe (MRS) Broth

Get tips on using Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027) to perform Protein Ladder Prestained

Products Abcam Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)

Get tips on using Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592) to perform Protein Ladder Prestained

Products Abcam Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592)

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