Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?
Get tips on using Staphylococcal Enterotoxin Reversed Passive Latex Agglutination Kit (SET- RPLA) to perform Cell Culture Contamination Detection Kit Bacteria
Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay bacteria - Clostridium sporogenes
Get tips on using TaqPath™ BactoPure™ Microbial Detection Master Mix, no Rox to perform Cell Culture Contamination Detection Kit Bacteria
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Get tips on using Lectin from Triticum vulgaris (wheat) to perform Western blot 1,4 β-N-acetyl-D-glucosamine - Triticum vulgaris Bacteria Biotin
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment