siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Get tips on using BrdU Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HT-29

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HT-29

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HCT-116

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HL-60

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MCF 10A