ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

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Get tips on using Multiplex CRISPR/Cas9 Assembly System Kit to perform CRISPR Human - Activation hATCB

Products Addgene Multiplex CRISPR/Cas9 Assembly System Kit

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Quantitative real-time PCR - Bacterial DNA

Products Qiagen QuantiFast Multiplex PCR Kit

Get tips on using QuantiNova Multiplex RT-PCR Kit (2500) to perform PCR Multiplex PCR - Viral

Products Qiagen QuantiNova Multiplex RT-PCR Kit (2500)

Get tips on using QuantiTect Multiplex RT-PCR Kit (1000) to perform PCR Multiplex PCR - Viral

Products Qiagen QuantiTect Multiplex RT-PCR Kit (1000)

Get tips on using QuantiTect Multiplex PCR NoROX Kit (1000) to perform PCR Multiplex PCR - Poultry DNA

Products Qiagen QuantiTect Multiplex PCR NoROX Kit (1000)

Get tips on using QuantiTect Multiplex RT-PCR NR Kit (1000) to perform PCR Multiplex PCR - Viral

Products Qiagen QuantiTect Multiplex RT-PCR NR Kit (1000)

Get tips on using DuoSet® ELISA Development Systems to perform ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α - -NA- Human -NA-

Products R&D system, Minneapolis, MN, USA DuoSet® ELISA Development Systems

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Discussions Problem in phase separation after using serum/plasma kit

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Human Blood / Serum / Plasma / Buffy coat

Get tips on using NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit to perform Cell Culture Contamination Detection Kit Virus

Products Thermo Fisher Scientific NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit

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