ChIP acH4 Rabbit Bovine

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - prostate cancer PPC1 cells

Get tips on using Anti-LC3 antibody produced in rabbit to perform Autophagy assay cell type - Human osteosarcoma cancer cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Human tracheobronchial epithelial cells (hTEC)

Get tips on using HighCell# ChIP kit to perform ChIP Rat - PCCL3

Get tips on using EZ-ChIP™ to perform ChIP Rat - NRK52E

Get tips on using EZ-ChIP™ to perform ChIP Human - HUVEC

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.