DNA Damage Assay Human bronchial epithelial cells (hBE)

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay Capan-2

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification MCF10A breast epithelial cells

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay MIA PaCa-2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human tracheal epithelial cells

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma