ROS assay cell type - human umbelical vein endothelial cells (HUVEC)

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 4 matching solutions for this experiment

Upstream tips
- 1-1.5×10^5 cells were platted.

- Cells were pre-treated with increasing concentrations of NS1 for 30 min at 37°C
Protocol tips
- CellROX Reagent at a final concentration of 2 μM and incubated for 30 min.
Upstream tips
- Cells were cultured in 96 well plate
Protocol tips
- Cells were treated with fluorogenic probe 2′,7′-dichlorodihydrofluorescin diacetate and incubated for 1h
Protocol tips
- Cells were incubated for 1h after adding fluorogenic probe.

- The assay was terminated by adding cell lysis buffer and incubating for 5 min.
Protocol tips
- Cells were treated with carboxy-H2DCFDA at a final concentration of 25μM in darkness at 37°C for 30 min.
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