PCR Quantitative real-time PCR

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using EZ DNA Methylation-Direct Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using Hot Start Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

Get tips on using SYBR GreenER™ qPCR SuperMix Universal to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using AmpliTaq Gold™ 360 DNA Polymerase to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using Ion AmpliSeq™ Library Kit 2.0 to perform PCR Multiplex PCR - Mammalian DNA

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.