Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Esophagus
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Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Colon
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Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD8+ T cells
Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD4+ T cells
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