Wound healing assay cell type rat

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat nucleus pulposus

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat primary hepatocytes

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - FaDu

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HaCaT

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using β-Gal Assay Kit to perform Reporter gene assay β-galactosidase substrates - PANC-1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.