siRNA / miRNA gene silencing Mouse Neuro 2a

Get tips on using Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit to perform ELISA Mouse - C-Reactive Protein/CRP

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Get tips on using Monoclonal Anti-Connexin-43 antibody produced in mouse to perform Western blotting CX43

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary mouse cortical neurons

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using CD273 (PD-L2) Antibody, anti-mouse, PerCP-Vio® 700 to perform Flow cytometry Anti-bodies Mouse - CD273/PD-L2

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Monoclonal Anti-γ-Tubulin antibody produced in mouse to perform Western blotting tubulin gamma